Protocols

Below are protocols that describe the steps involved in a CRISPR-Cas9 screen (Based on Wit et al. (2023)):

(NEEDS TO BE UPDATED!):

1. Creation of Cas9 expressing cell line (Download)

2. Cas9 activity assay using flowcytometry (Download)

3. sgRNA library virus production and tritrations (Download)

4. CRISPR-Cas9 screening (Download)

References

1. Wit, N. et al. A histone deacetylase 3 and mitochondrial complex I axis regulates toxic formaldehyde production. Science Adv. 9 (20), eadg2235 (2023)